Efficient pre‑treatment for pancreatic cancer using chloroquine‑loaded nanoparticles targeting pancreatic stellate cells

Pancreatic stellate cells (PSCs) play a key role in desmoplastic stroma, which is characteristic of pancreatic ductal adenocarcinoma (PDAC), and they also enhance the malignancy cancer cells. Our previous study reported chloroquine's mitigating effects on PSC activation; however, drug known to induce adverse clinical practice. The present aimed reduce chloroquine doses develop useful pre‑treatment that targets PSCs using nanoparticles. Poly lactic‑co‑glycolic acid (PLGA) nanoparticles were used as carriers loaded with indocyanine green (Nano‑ICG) or (Nano‑CQ). Tumor accumulation Nano‑ICG was evaluated an vivo imaging system. chloroquine, Nano‑CQ and/or chemotherapy gemcitabine investigated orthotopic xenograft mouse model. selectively accumulated tumors persisted therein for over 7 days after administration. Additionally, peritoneal metastasized regions, but not liver, kidney normal tissues. reduced density activated at lower significantly restrained tumor progression combination gemcitabine. In conclusion, PLGA nanosystem successfully delivered tumors. efficiently activation may be promising novel strategy PDAC.8